1.
Studies On Gentamicin Toxicity In Broiler Chickens
by Muhammad Shah Rukh | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Omer | Prof. Dr | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2004Dissertation note: This project was designed to evaluate the toxic effects of gentamicin sulphate at different dosage levels in broiler chicks. The experiment was conducted in two phases. During phase I one hundred broiler chicks were divided into A,B, C and D groups having twenty five birds in each and were injected gantamicin sulphate in the form of gentalin 5% intramuscularly at the rate of 5mg/kg, lOmg/ kg, 20mg /kg in groups A,B and C twice daily for four days respectively while group D kept as control. During phase II twenty five chicks were divided into groups E,F,G,H and I having five birds in each. Birds in groups E and F were injected gentalin 5% intramuscularly at the rate of 40mg/kg and 80 mg/kg while gantamicin sulphate injected in the form of Aagent 10% at the rate of 40 mg / kg and 80 mg/kg to groups G and H twice daily for four days respectively and the birds of I group were kept as control.
Blood samples (3m1) were collected from all medicated and un-medicated groups before and after medication on different days and serum was separated for the measurement of different biochemical parameters. Post-mortem examination of all birds were performed at the end of experiment. Post-mortem examination revealed no abnormality in any bird during phase I (normal organs) while during phase II abnormalities such as congestion, inflammation of the kidney and necrosis, discoloured livers were recorded except control group. Six birds died during experiment in phase 11(2/10 at 40mg/kg and 4/10 at 80mg/kg dose). The mortality rates at 40 mg/kg and 80 mg/kg were 20 and 40% respectively. An increase in Aspartate amino Transferase was observed in medicated birds during phase I while a significant increase in Aspartate amino Transferase, Alanine amino Transferase, uric acid and creatinine were recorded in medicated birds during phase II. From this study it is concluded that broiler birds tolerated gentamicin upto 20mg/kg while acute toxic effects at the dose of 40mg/kg and 80mg/kg of gentamicin were recorded in broiler chicks. No sign of visceral gout were recorded in any bird. There was no difference in the toxicity of two different preparations of gentaniicin.
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2.
Bioavailability Of Amoxicilin Trihydrate In Broiler Chickens Administered Through Drinking Water
by Amir Bakhsh | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Ovais Omer | Prof. Dr | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2004Dissertation note: Bioavailability of two oral commercial formulations of amoxicillin trihydrate i.e. oracin-50 (Mediexcel, Pharmaceuticals (Pvt.) Limited Pakistan) and Bioxil-20 (Bioceutica, µµchickens. The birds were divided into two groups A and B and than six replicates of each group were made. The birds were reared upto 30 days. Group A was medicated by Oracin-50 and group B was medicated by Bioxil-20. The medication was continued for three consecutive days and blood samples were collected before and after medication as per schedule. The plasma level of amoxicillin were determined by microbiological assays. The concentrations versus time data were plotted on logrithmic paper and various bio-availability parameters were determined.
Peak concentrations of both oral preparations appeared with in two hours. However Oracin-50 peak concentration revealed 5.26±1.605µg/ml which was not significantly higher than 4.71±0.142ig/ml as depicted by Bioxil-20.
Mean ±SEM of the area under curve (AUC) for Oracin-50 was 296.783±6.331µg. hr/ml and 259.26±4.866 µg.hr/ml for Bioxil-20 statistical analyses revealed no significant different minimum concentration of Oracin-50 revealed 2.550±O.0763µg/ml while 2.450±O.07637µg/ml as depicted by the bioxil-20.
Both the preparations took the same time (2 hour) to attain the peak level of the plasma concentration. The present study also revealed that amoxicillin is not much stable in water. Oracin-50 decreased 34% and Bioxil-20 decreased 35% in 72 hours There was no significant difference (P<O.05) in the stability of both groups. The comparative study of the two Pharmaceutical preparations Oracin-50 (Mediexcel Pharmaceutical Pakistan) and Bioxil-20 (Bioceutica Pharmaceutical Pakistan) revealed that there was no significant difference (P<O.05) between the means of two groups. The statistical evaluation revealed bio-equaleancy between two drugs.
Amoxicillin is broad spectrum antibiotic, its bioavailability is better than other antibiotic. Its MIC is also very low (O.25igIml) but its half 1fe is short. So its repeated doses should be given. As it is somewhat unstable, in case of drinking water. The medicated water should be changed get the maximum efficacy.
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3.
Preparation And Evaluation Of Rabbit Anti-Buffalo Immunoglobulin Antibody Peroxidase Conjugate
by Muhammad Babar | Prof.Dr.Muhammad Ashraf | Mr. Shahid Abbas | Prof. Dr | Faculty of Biosciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: Enzyme linked immunosorbent assay (ELISA) is one of the most sensitive rapid and reliable techniques for diagnosis of infectious diseases. For execution of ELISA, antibody-peroxidase conjugate is the fundamental reagent. Turnip peroxidase was purified from turnips, that includes homogenization, inactivation of catalase, ammonium sulphate precipitation and size exclusion chromatography on Sephadex G-25-80. The purified peroxidase had Rz value of 1.7, total protein 0.9 mg/ml and total enzyme activity 36152 units/liter. The buffalo serum Ig-G was fractionated using 40 percent final concentration of ammonium sulphate followed by anion exchange chromatography. The salt fractionated serum globulins (10 ml) was depleted of its Ig-G in less than 25 minutes on DEAE cellulose packed column followed by suitable elution. The Ig-G solution (1.0 gm/dl) was mixed in four times volume of oil base (Liquid paraffin and emulsifiers). Rabbits were primed and boosted (0.25ml/: subcut) with buffalo Ig-G antigen with 21 days interval. The immune serum was harvested on 21 days post-boosting. The serum contained 2048 agar gel precipitation AGP units and 10,000 ELISA units. Rabbit anti buffalo Ig-G was purified with salt precipitation followed by anion exchange chromatography. The peroxidase was linked with the rabbit anti-buffalo Ig-G using the sodium metaperiodate. The conjugate was titrated against buffalo Ig-G and working dilution for execution of ELISA was 1: 2000.
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4.
Biological Health Markers Of Pregnant Crossbred Cows
by Shaista Abbas | Prof. Dr. Ijaz Ahmad | Dr. Habib-ur-Rehman | Faculty of Biosciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2010Dissertation note: Pregnancy is a period characterized by an increase in metabolic demand owing to changes in female physiology and the requirements for growing fetus. The changes that take place in various body systems during pregnancy result in an increased oxygen demand and changes in energy substrate utilization by dam, especially in the feto- placental unit. Pregnancy, though a physiological state, is a stressful condition that favors the oxidative stress and results in an imbalance between pro-oxidant and antioxidant systems of the body leading to potential damage. Metabolic demands associated with late gestation, parturition, and initiation of lactation has been supposed to increase the production of reactive oxygen species (RGS).
The current study was undertaken to elucidate the dynamics of different biological health and metabolic markers in crossbred cows at different stages of pregnancy and non-pregnant stage. The study was carried out on 40 (age range between 4 and 11 years) clinically healthy crossbred cows (Sahiwal + Holstein-Friesian). The animals were divided into four groups; first trimester, second trimester and third trimester of pregnancy, and non-pregnant, with 10 cows in each group. The results revealed that serum total oxidants, ceruloplasmin oxidase and tri- iodothyronine_ concentrations were higher (P<0.05) during third trimester of pregnancy compared to non-pregnant crossbred cows. Serum arylesterase concentration was lower (P<0.05) during second and third trimesters of pregnancy compared to non-pregnant stage. The concentration of serum total homocysteine was higher (P<0.05) during third trimester compared to first and second trimesters of pregnancy. The concentrations of serum total antioxidants, paraoxonase, total thyroxine, aspartate aminotransferase and alanine aminotransferase were not different during pregnancy and non-pregnant stage in crossbred cows.
In conclusion, the biological health markers specially total oxidant status, aryl esterase, ceruloplasmin oxidase, total homocysteine and tri-iodothyronine of pregnant crossbred cows can provide useful information about the progression of pregnancy and can also be used as management tool for the improvement of the health status during pregnancy.
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5.
Comparision Of Mineral Profile In Lactating And Non-Lactating Nilli-Ravi Buffalo
by Mohammad Akram | Prof. Dr. Saghir Ahmed Jafri | Dr. Muhammad Iqbal | Prof. Dr. Rashid | Faculty of Biosciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Nih Ravi is the well established breed of buffalo in Punjab. There are about 140 million buffaloes in the World out of which the largest buffalo population is in India, China and Pakistan. It is a multipurpose animal and fulfils milk and meat demand of mankind. In Pakistan it contributes about 74% of total milk yield. The annual rate of increase in buffalo population is 2.5% and increase in milk production is 3.5% in India and Pakistan. To fulfil the increasing demand of milk and meat, there is an immense need to improve the health status and production potentials of dairy animals. This can he achieved only if we are abreast with physiological norms, the disturbance of which lowers productivity. There are numerous problems of health, reproduction and milk production of Nih Ravi buffalo. Despite this very little information is available on blood chemistry of buffalo belonging to different agro-clirnatic conditions. There are various factors which play important role in their rate of production and minerals are one of those factors. Imbalances of minerals, produces deterimental effect on the performance of livestock e.g. Mineral imbalances lead to syndromes manifested in form of retarded growth, low production, late maturity, wasting disease, non infectious abortion, infertility, silent heat, retained placenta, dystokia and prolapse of vagina and uterus. To cope with the situation more research is required on the subject to locate the extent of mineral imbalances. The conclusive results will produce direct or indirect effect on the production and reproductive performance of livestock. The study is therefore planned to find out the serum mineral profile of lactating and non lactating buffaloes from different areas of Punjab and the effect of stage and number of lactation on mineral profile and to find the correlation of serum mineral profile with prolapse of vagina and uterus.
To determine the mineral profile in lactating and non lactating Nih Ravi buffaloes 200 blood samples were collected from Okara, Jhelum and Lahore/Sheikhupura. The serum was separated from blood by centrifugation. Serum was analyzed for sodium, potassium, chloride, calcium, magnesium, phosphorus and iron on spectrophotometer. The data thus obtained was subjected to statistical analysis by using analysis of variance and the differences in means were tested by using (L.S.D. test) least significant diference test.
The mean values of serum mineral in lactating and non lactating buffaloes on overall basis were subjected to statistical analysis and found non significant differences. However serum magnesium levels were found to be significant in lactating and non lactating buffaloes.
The means values of serum minerals in buffaloes of various districts were subjected to statistical analysis by using analysis of variance and L.S.D. test and significant differences were found between districts (P <0.05) for sodium, potassium, magnesium, phosphorus and iron, while non significant differences were found for calcium and chloride (P>0.05).
The mean values of serum mineral in lactating and non lactating buffaloes on the basis of lactation number were subjected to analysis of variance and L.S.D. test and found non significant differences for serum sodium, potassium, chloride, calcium, magnesium and iron, while serum phosphorus showed singificant differences on the basis of lactation numbers and stage of lactations.
The differences in the mean values of serum sodium, potassium, chloride, magnesium and phosphorus were found to be significant. While calcium and iron showed non significant differences within distircts.
The serum sodium showed deficiency in Lahore/Sheikhupura district while serum potassium, chloride, calcium, magnesium and iron values were found in normal range in all districts. However serum phosphorus level were slightly higher in buffaloes of all the districts.
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6.
Alleviation Of Cyclic Heat Stress In Broilers By Dietary Supplementation Of Mannan-Oligosaccharides And Lactobacillus-based Probiotic
by Muhammad Umar Sohail | Prof. Dr. Ijaz Ahmad | Prof. Dr | Prof. Dr. Hibib ur Rehman | Faculty of Biosciences.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The antiviral activity of plants Silybum marianum (seeds), Chenopodium album (whole plant) and Nigella sativa (seeds) were evaluated against Peste des petitis ruminants virus (PPRV) and Foot and Mouth Disease virus (FMDV) in this study. Methanolic extraction of these plants was done by using Soxhlet apparatus and extracts were dried by using rotary evaporator. Six dilutions of each extracts 100, SO, 2S, 12.S, 6.2S, 3.12~g/ml were made in distilled water. Vero cells were infected by PPRV and BHK-21 by FMDV respectively. The herbal extracts assays of antiviral and cytotoxic were carried out in cell culture plates. Each well of 96 well cell culture plate were seeded with 104cell/ml of cell suspension. Cell counting was performed by hemocytometeric method. Positive and negative controls for antiviral and cytotoxic assay were also used, incubated the 96 well cell culture plates at 37°C for 4 days. After this incubation, MTT [3-(4,S-dimethylthiazol-2-yl)- 2,S-diphenyltetrazolium bromide] colorimetric assay were used for the determination of their quantification. Endpoint of this assay was considered in terms of cell survival percentage. Results were compared for qualitative variables using Chi-square technique and quantitative variables by linear regression analysis. 1 OO~g/ml and SOIlg/ml concentrations of Chenopodium album showed cell survival percentages of 87.9% and 86% respectively in PPRV and all six test dilutions of same plant showed no cytotoxicity for Vero cells. IOuug/ml and
SO~g/ml concentrations of Chenopodium album showed cell survival percentages of 88.5% and 87.2% respectively in FMDV and all six test dilutions of same plant showed no cytotoxicity for BHK-21 cells. Two concentrations of Nigella sativa 50!J. glml and 25!J. glml showed prominent cell s urvival of 85% and 84% respectively in PPRV and only one concentrations l Ouug/ml were found cytotoxic.Two concentrations of Nigella sativa 50uglml and 25!J.glml showed prominent cell survival of 79% and 77% respectively in FMDV and only one concentrations IOuug/ml were found cytotoxic. Only IOuug/ml of Silybum marianum has shown cytotoxicity and 50!J.glml and 25!J.glml shown prominent antiviral activity 91% and 85% respectively in PPRV. In FMDV l Otlug/ml of Silybum marianum has shown cytotoxicity and 50!J.glml and 25!J.g/ml shown prominent antiviral activity 93% and 91 % respectively. The results of present study are helpful in the treatment of Peste des petitis ruminants and Foot and Mouth Disease.
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7.
Serum Biochemical Metabolites, Electrolytes And Minerals Profiling In Heat Stressed Donkeys (Equus Asinus)
by Naz Fatima | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Habib-ur-Rehman | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Background
In developing countries, equines are used as a companion animal, for transportation, carrying goods, pulling carts and to ride. Heat stress (HS) is the major problem of tropical and subtropical region that adversely affects the performance of livestock by altering the physiological indices.
Objective
The current study is designed to report the dynamics of different physiological indices including serum electrolytes, minerals, biochemical metabolites, hepatic enzymes and thyroid hormones in heat stressed (HSd) donkeys (Equus asinus).
Materials and Methods
A total of 20 donkeys were involved (10 healthy and 10 clinically diagnosed as HSd), irrespective of their gender and age, in the current study. The health of the donkeys was evaluated by observing vital signs including body temperature, respiratory rate, heart rate and hematocrit. Blood was collected from each animal during summer season in August and serum was harvested by centrifugation, of clotted blood, at 4,000 rpm for 20 minutes. Serum samples were stored at -20°C for analysis. Serum electrolytes and minerals were determined using flame photometry and atomic absorption spectrophotometer, respectively, after wet digestion of serum. Serum biochemical metabolites and hepatic enzymes were evaluated using commercial kits, spectrophotometrically. Serum thyroid hormones concentrations were determined using commercial ELISA kits. Normal distribution of the data was confirmed by Shapiro-Wilk test. Data was analyzed using Independent Student's t-test by employing Statistical Package for Social Science (SPSS). Data was presented as means ± SE. Differences were considered significant at P < 0.05.
Results
Results revealed significantly higher heart rate, respiratory rate, rectal temperature and hematocrit in heat stressed donkeys. Results revealed that serum sodium and potassium concentrations were significantly lower and serum calcium, magnesium and iron concentrations were significantly higher in heat stressed donkeys. However, there was no significant difference in Na/K ratio and serum chloride in HSd donkeys. Results revealed significantly higher serum total proteins, albumin, globulins concentrations, A/G ratio, LDL-cholesterol levels, T3, T4, AST, ALT, and ALP activity and homocysteine concentrations in heat stressed donkeys. However, serum HDL-Cholesterol and triglycerides levels were significantly lower. No significant difference in concentrations of serum glucose and cholesterol was observed.
Conclusion
Heat stress decreases electrolytes concentration and increases homocysteine and LDL-cholesterol levels that may account for increased risk of cardiovascular manifestations.
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8.
Serum Biochemical Metabolites, Electrolytes And Minerals Profile Durings Different Lactations In one-Humped Lactating Camels (Camelus Dromedarius)
by Anum Khushal | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Habib-ur-Rehman | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Background
Lactation is characterized by change in metabolic demands of body that induces stress in lactating animals. During lactation the body reserves of nutrients, electrolytes and minerals are lost in milk leading to depletion of reserves. Therefore, lactating animals may suffer from deficiency of nutrients, electrolytes and minerals.
Objective
The objective of the study was to report whether lactations alter the serum biochemical metabolites, electrolytes and minerals profile of one-humped lactating camels or not.
Materials and Methods
The study involved 25 healthy, one-humped lactating camels (Camelus dromidarious) selected randomly from the desert area of Bhakkar (Punjab, Pakistan). Camels were divided into five groups (each having five camels) on the basis of lactations. The study involved camels in first, second, third, fourth and fifth lactations.
Ten milliliter blood was drawn from jugular vein using sterilized needles and plane syringes. Blood samples were allowed to clot at room temperature and centrifuged at 3,000 rpm for 15 minutes. Serum was collected and stored at -20ºC till analysis. Serum biochemical metabolites and hepatic enzymes were evaluated spectrophotometrically using commercial kits and serum thyroid hormones were determined using commercial ELISA kits. Serum electrolytes and minerals were determined using flame photometery and atomic absorption spectrophotometery, respectively, after wet digestion of serum. Data was analyzed using one way analysis of variance by SPSS and significance was set at P < 0.05.
Results
Serum concentration of glucose, total proteins, albumin and globulins, triglycerides, HDL-cholesterol, LDL-cholesterol, uric acid, and thyroid enzymes did not differ among all the five lactation stages. Serum cholesterol concentration was significantly higher during second lactation compared with the first, third, forth and fifth lactations. Serum aspartate aminotransferase concentration was significantly higher during first lactation compared with the fourth lactation. Serum alanine aminotransferase concentration was significantly higher during fifth lactation compared with the first lactation. Serum concentration of minerals like calcium, magnesium, iron, manganese and selenium did not differ during any lactation. Similarly, serum electrolytes including sodium and potassium also did not differ between subsequent lactations.
Conclusion
Lactations have not much pronounced effects on the serum biochemical metabolites, electrolytes and minerals profile of one-humped lactating camels.
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9.
Evaluation Of Different Extenders For The Cryopreservation Of Buffalo Bull (Bubalus Bubalis) Semen
by Dawar Hameed Mughal | Prof. Dr. Ijaz Ahmad | Prof. Dr. Muhammad Aleem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Buffalo is playing an important role in our country's economy by producing milk, meat and draught power. Genetic potential of low producing animals can be improved by using artificial insemination technology. Unfortunately, less number of elite bulls are available and low fertility rate of buffalo by using cryopreserved semen has been obtained. Semen is exposed to osmotic and oxidative stresses during processing, cryopreservation and thawing before insemination. Fertilizing ability is lost due to spermatozoa damage and it ultimately results in poor conception rates in buffalo. In order to protect spermatozoa from these stresses and improve fertility in buffalo, five osmotic pressure based concentrations of three extenders i.e. Citrate egg yolk extender (CEYE), Tris egg yolk extender (TEYE), and Lactose egg yolk extender (LEYE) were prepared by varying the quantity of the solutes to obtain an osmotic pressure of 255, 265, 275, 285 and 295 mOsm/kg. Osmotic pressure was measured by an osmometer.
In the first experiment, equal volume of semen obtained from four Nili-Ravi buffalo bulls was pooled and used to study the effects of osmotic pressure on post thawed semen characteristics. For this purpose, three basic media: citrate fructose media, tris citric acid fructose media and lactose media were prepared and divided each media in to five equal parts to maintain osmotic pressures of 255, 265, 275, 285 and 295mOsm/kg. These basis media were stored in a biomedical freezer, which were later used in preparing three semen extenders i.e. Citrate egg yolk extender (CEYE), Tris egg yolk extender (TEYE), and Lactose egg yolk extender (LEYE). During each collection, fifteen extenders (each of three extenders having five osmotic pressures i.e. 255, 265, 275, 285 and 295mOsm/kg) were used to extend the semen. After freezing, semen characteristics like sperm motility rate, viability rate, acrosomal integrity rate, plasma membrane integrity (PMI) rate, MTT reduction rate, sperm DNA integrity rate and lipid peroxidation were noted. Post thaw sperm motility rate in (%) CEYE was significant (P<0.05) at 295mOsm/kg compared to 255, 265 and 275mOsm/kg. However, sperm motility rate of different osmotic pressures of TEYE and LEYE was non-significant (P>0.05). Sperm viability rate (%) was non-significant (P>0.05) in all three extenders. Sperm acrosomal integrity rate was non-significant in CEYE and LEYE. However, it was significant (P<0.05) at 265, 275 and 295mOsm/kg in TEYE. Sperm PMI rate, MTT reduction rate, sperm DNA integrity rate and lipid peroxidation were non-significant (P>0.05) in CEYE, TEYE and LEYE.
On the basis of the individual and overall comparison of different semen characteristics of three extenders and their osmotic pressures, LEYE with 295mOs.kg was considered to be continued in the next experiment to upgrade the extender by adding taurine (TA) at 0.0, 30, 50 and 70 mM and trehalose (TR) at 0.0, 20, 40, 60 mM concentration. Semen collection, processing, freezing etc were done as per experiment-1 and same post thaw tests were carried out. Post thaw sperm motility rate was significantly (P<0.05) higher at TA-0.0 and TA-20mM and all concentration of TR. Sperm viability rate, acrosomal integrity rate, PMI rate, MTT reduction rate and lipid peroxidation at different concentrations of TA and TR were recorded non-significant (P>0.05). However, sperm DNA integrity rate was significant (P<0.05) higher at TA-0.0 and TR-0.0mM.
On the basis of comparison of different semen characteristics under various concentrations of taurine or trehalose, supplemented in semen extenders. Concentration of TR-70mM was considered to be continued in the next experiment to test fertility of the optimized extender.
Semen straws of LEYE supplemented with TR-70mM were used to inseminate the 50 buffaloes in heat (Supplemented group), while, traditionally used tris based buffalo bull semen extender was used (control group) to compared pregnancy rate (PR) of this experiment. Pregnancy rate in control and supplemented group was 38 and 54% respectively, which was statistically non-significant (P>0.05).
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10.
Determination Of Various Physiological Indices In Dogs Induced By Antipiroplasm Drug
by Tanzila Bashir | Dr. Imtiaz Rabbani | Prof. Dr | Prof. Dr. Ijaz Ahmad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1644,T] (1).
11.
Response Of Dietary Yeast Supplementation In Chronic Heat Stressed Broilers On Growth Performance and Organs Development
by Islam Zeb Khan | Dr. Muhammad Shahbaz Yousuf | Prof. Dr. Habib-ur-Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1676,T] (1).
12.
Seasonal Variations In The Testicular And Seminal Characteristics Of Cholistani Bulls
by Umer Farooq | Prof. Dr. Ijaz Ahmad | Dr. Hafsa Zaneb | Prof. Dr. Habib Rehman | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: All the indigenous cattle of Pakistan belong to Zebu (one-humped) cattle (Bos indicus). There are 15 recognized indigenous breeds of cattle which constitute 43% of the total cattle population in the country. Hefty work has been documented on many aspects of Sahiwal and Red Sindhi breeds of cattle in their respective local climates which have rightly gained them an international fame as being the vital tropical milk breeds of Pakistan. However, scanty work has so far been reported on reproductive indices of other indigenous cattle breeds of Pakistan such as Dajal, Cholistani etc under their local climatic conditions, and their potential still remains unearthed. Hence, it is the dire need of time to comprehend the urgency of protection and propagation of the indigenous cattle breeds of Pakistan at every front.
Following the global lead, an effort has been made through the present study to highlight a formerly neglected indigenous cattle breed of Pakistan- Cholistani- being reared by the nomadic herders of the Cholistan desert, Pakistan. The study was executed with a specific objective to assess baseline data, seasonal variations and correlations between various non invasive, readily measurable reproductive [age, body weight (BW) and orchidometric parameters viz. scrotal circumference (SC), scrotal skin fold thickness (SSFT), average testicular length (Avg L), average testicular width (Avg W) and paired testicular volume (PTV)], seminal (fresh semen and seminal plasma) and haematobiochemical attributes of Cholistani AI bulls (n=06). Keeping in view the prevailing climatic conditions of the study area, 4 seasons of 2 months duration each were defined as i) stress free autumn (October-November), ii) stressful winter (December-January), stressful dry summer (May-June) and iv) stressful wet summer (July-August).
The overall mean values (± SEM) for age, BW, SC, SSFT and PTV in the present study were 92.83±2.63months, 527.55±4.5kg, 35.39±0.28cm, 1.03±0.02cm and 851.84±16.34cm3, respectively. The BW was recorded to be significantly lower (P<0.05) during the stress free autumn (507.08±8.26kg), however no significant difference was found in the remaining stressful seasons of winter, dry and wet summer. The SSFT was significantly higher (P<0.05) in stress free autumn (1.07±0.04cm) and winter (1.13±0.05cm) being followed by that in wet summer (0.97±0.02cm) and dry summer (0.94±0.03cm). SC and PTV were found to be constant during all the study seasons with no significant variation. Significantly positive correlations (P<0.01) were noticed between various traits.
The overall mean values (± SEM) for ejaculatory volume, number of ejaculates per bull, mass motility, individual sperm motility, number of dozes frozen per bull, sperm concentration, live sperm, morphologically normal sperm and those with acrosome integrity in the present study were 4.92±0.14mL, 1.89±0.02, 2.26±0.06, 63.51±1.03%, 208.26±8.42, 989.73±27.4million/mL, 86.74±0.55%, 86.34±0.51% and 86.64±0.48%, respectively. In general, all the seminal attributes revealed significantly (P<0.05) better results in stressful seasons as compared to stress free season, with the exception of number of ejaculates per bull which was not affected significantly by seasons.
In terms of semen production, a total of 39778 doses of 0.5 mL straws were frozen during the study period. Significantly higher number of doses per bull (P<0.05) was frozen during dry (245.37±17.84) and wet summer (250.37±17.75) as compared to autumn (191.4±14.22) and winter (143.92±11.25); the difference between the latter two seasons was also significant, while it was non significant between the former two seasons
Regarding the subjective analysis of spermatozoa, higher (P<0.05) mean values were recorded for all three parameters (live, morphologiclly normal and acrosome intact sperm) during stressful winter and dry summer as compared to wet summer and autumn, the difference between the former two seasons was, however, non significant. Moreover, mean values for these attributes were significantly higher during wet summer as compared to autumn. Amongst the physical attributes of semen, only mass motility revealed a significantly positive (P<0.01) correlation with individual sperm motility (r 0.619). However, regarding the subjective analyses, live sperm had a positive correlation with normal (r 0.848) and with acrosome integral sperm (r 0.790).
In seminal plasma chemistry, the overall mean values (± SEM) for Na+, K+, cholesterol and glucose recorded in the present study were 236.41±4.2, 142.95±3.81, 152.02±3.17 and 127.22±3.65 mg%, respectively. Amongst these, Na+ was significantly higher (P<0.05) during dry summer (270.35±8.89mg%) whereas K+ was higher in dry summer (169.1±5.54mg%) and winter (154.80±6.29mg%). Cholesterol and glucose were found to be significantly elevated (P<0.05) during dry summer season being 173.7±4.24 and 147.95±7.74mg%, respectively. Na+ had a significantly positive (P<0.05) correlation with glucose (r 0.232). Similarly, positive (P<0.01) correlations were seen for K+ with Na+ (r 0.341) and cholesterol (r 0.390).
The overall mean values (± SEM) for Hb, TEC, PCV, MCV, MCH and MCHC recorded for the Cholistani AI bulls in the present study were 12.24±0.15mg/dL, 6.28±0.82×106/µL, 37.3±0.4%, 59.77±0.61fL, 19.58±0.2pg and 32.89±0.33g/dL, respectively. Regarding the white blood cell values, the overall means (± SEM) for TLC, neutrophils, lymphocytes, monocytes and eosinophils were 7.58±0.17×103/µL, 40.45±1.05%, 53.2±0.85%, 4.05±0.47% and 2.3±0.26%, respectively. DLC, in the present study, revealed that the lymphocytes were the predominant leukocytes followed by neutrophils. No significant effect (P<0.05) of season was noticed on any of the red blood cell values studied. However, TLC amongst the white blood cell values was found to be significantly higher in dry summer (8.16±0.28×103/µL).
The overall mean values (± SEM) for blood serum NA+, K+, cholesterol and glucose recorded for the present study were 130.73±0.91, 7.37±0.11, 82.8±1.52 and 75.22±2.04mg%, respectively. Correlation analysis revealed significant positive correlations between mass and individual sperm motility, morphologically normal and live sperm and sperm acrosome integrity and live sperm (P<0.01). Among biochemical attributes of seminal plasma, Na+ and K+, cholesterol and K+ and glucose and Na+ were correlated (P<0.01). Only seminal plasma cholesterol was positively correlated with ejaculatory volume (P<0.01).
An overall mean value of 5.81±0.32ng/mL was recorded in the present study for testosterone being non significantly effected by seasons. All its correlations with all attributes studied were found to be non significant.
In conclusion, various physical and biochemical attributes of Cholistani bull semen were better in stressful seasons as compared to stress free season. The acceptable quality of semen of Cholistani bulls recorded during wet/dry summer in the present study is an indicative of the fact that this breed has an innate ability of being well adapted to the harsh, hot desert climate. Furthermore, the present study revealed that the Cholistani breeding bulls have an amazing tendency to maintain most of their haematobiochemical parameters at a near constant level without showing much variation during stress free or stressful times.
Availability: Items available for loan: UVAS Library [Call number: 1739,T] (1).
13.
Response Of Cryopreserved Cholistani Bull Semen To Butylated Hydroxytoluene Inclusion In Semen
by Abaid-ur-Rehman Cheema | Prof. Dr. Ijaz Ahmad | Dr. Dawar | Dr. Imtiaz Rabbani.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1745,T] (1).
14.
Effect Of Anti-Stressor Supplement On Varous Physiological Markers Of Broiler Chicks Under Chronic Heat Stress
by Madeeha Wajid | Dr. Imtiaz Rabbain | Prof. Dr. Habib-ur- Rehman.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1801,T] (1).
15.
Assessment Of Optimal Level Of Butylated Hydroxytoluene For Short Term Storage Of Broiler Breeder Semen
by Sikander Abbas | Prof. Dr. Ijaz Ahmad | Dr. Ali Ahmad | Dr. Muhammad Shahbaz Yousaf.
Material type: ; Format:
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not fiction
Publisher: 2013Dissertation note: Short term Preservation of broiler breeder semen is an unnatural method in which spermatozoa faces oxidative stress, osmotic and thermal shocks. This affects preserved semen quality resulting in low conception rates. In order to overcome oxidative stress, Butylated Hydroxytoluene was used as antioxidant in poultry semen during short term preservation at 4ºC for 48 hours.
Keeping in view the significance of BHT as a potent antioxidant, the present study was conducted to determine the significance of BHT inclusion in preserved Hubbard broiler breeder semen for reduction of oxidative stress. In the current study, semen from six (n=6) healthy broiler breeder was collected by abdominal massage and butylated hydroxytoluene (BHT) was added to the semen @ 0.5, 1.0, 1.5 and 2.0 mM. One group was kept control and no BHT was added to that group. The routine quality evaluation of semen for motility and concentration was made, extender was added and then semen was stored at 4°C for 48 hours.
The parameters evaluated were percentage motility, plasma membrane integrity (HOST), viability (Live/Dead) and DNA integrity (Acridine orange assay). Seven samples were taken from each concentration of BHT and thawed individually in water bath at 37°C for 30 seconds and evaluated for quality parameters. In conclusion, the addition of 1 mM BHT to semen extender improved marginally the broiler breeder spermatozoa motility, viability, plasma membrane integroty and DNA integrirty at different period of time.
In controled group the quality of semen after preservation was poor due to oxidative stress which will effect the fertility rate. The Butylated Hydroxytoluene has antioxidant properties, so inclusion of BHT in preserved Hubbard broiler breeder semen was tested in our research.
Availability: Items available for loan: UVAS Library [Call number: 1808,T] (1).
16.
Effect Of Age And Gender On Hematological And Biochemical Parameters Of Beetal Goat And Lohi Sheep
by Sidra Shafique | Dr. Imtiaz Rabbani | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Khalid.
Material type: ; Format:
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drama
Publisher: 2014Dissertation note: Small ruminants are one of the domestic animals of the inhabitants of Pakistan. In semi-arid and arid areas goat and sheep provides the main means of survival and security. So the proper health management of animals is must require for their survival. Hematlogical and biochemical tests are widely used for the diagnosis of serious animal diseases which can lead to economics losses in animals like reduced fur, wool and milk production. A large number of factors such as sex, age, gender, breed, illness, stress, seasonal variation and nutrition can affect the pattern of these clinical values. Our hypothesis was to evaluate the effect of age and gender on hematological and biochemical indices of Beetal goat and Lohi sheep in Ravi campus Pattoki UVAS.
One hundred and twenty blood samples (60 Goats and 60 sheep) were taken and divided on the basis of their age and gender (0-3 months, 9-12months and 18-20 months). Complete blood counts were performed by hematology analyzer (Abacus junior vet. Serial # 130076, Diatron GmbH wein, Austria) at W.T.O, UVAS. The biochemical parameter included albumin, globulin, cholesterol, triglycerides, total protein, urea AST and ALT were determined by commercially available kits (Biogene® and Crescent®). The arithmetic means (± SE) of hematological and biochemical parameters in different groups were calculated. The arithmetic means (± SE) of hematological and biochemical parameters in different groups were calculated. The collected data were subjected to two way analysis of variance through statistical package SPSS version 13 to evaluate mean differences in hematological and blood biochemical parameters of different age groups. Difference among the means of groups with p<0.05 was considered as significant through LSD test (Steel et al.1997).
In this study age and gender had a remarkable influence on the pattern of hematological and biochemical values of both sheep and goat. The RBCs, Hb, PCV, MCV, MCH, MCHC, WBCs and lymphocytes counts found significantly (p<0.05) higher in animal of age 18 to 20 months as compared to other groups in both species, While MCHC, monocytes and granulocytes found non-significantly (p>0.05) different. Male showed statistically (p<0.05) higher RBCs, Hb, PCV and MCV values as compared to female. The glucose, cholesterol, triglyceride, urea, AST, ALT, Total protein, albumin and globulin were found significantly (p<0.05) different by the aging of animal. Gender had no significant (p>0.05) influences on biochemical parameters of both sheep and goat.
Conclusion
Based on these findings age and gender had significant influence on hematological and biochemical parameters. In general veterinary practices, body weights are usually considered for therapeutic and nutritional strategies. Often too young animals are treated with more caution. In conclusion it can be mentioned that not only body weight but also age of the animals should be considered for devising therapeutic and nutritional strategy in livestock.
Availability: Items available for loan: UVAS Library [Call number: 1814,T] (1).
17.
Effects Of Terminalia Chebula On Blood Biochemical Profile And Pancreatic Tissue In Diabetic Rats.
by Daniyal Kazmi | Dr. Imtiaz Rabbani | Prof. Dr. Habib-ur-Rehman.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1834,T] (1).
18.
Antiangiogenic Activity Of Extracts Of Vinca Rosea (Catharnathus Roseus) Using Cam Assay
by Ijaz Ahmed | Prof. Dr. Habib-ur-Rehman | Dr. Imtiaz Rabbani | Syed.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1835,T] (1).
19.
Effects Of Supplementation Of Catharanthus Roseus And Probiotic On Hematologica Profile And Immune
by Shumaila Zia | Prof. Dr. Ijaz Ahmad | Dr. Asif Nadeem | Dr. Imtiaz Rabbani.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Background: The drugs which are used for curing diabetes caused adverse effects and complications to liver and kidney functions. Besides the side effects of drugs, no drug completely maintained and controlled the complications of diabetes. Due to the undesired side effects and partially compensatory treatments for metabolic disorders demands to formulate latest plant based therapies for the treatment of diabetes. There is also a need to establish the treatment which avoids the use of insulin injection.
C. roseus possessed anti-microbial, antifungal, antioxidant, anticancer antiviral and anti-hyperglycemic activities. The present study will be conducted to evaluate the change in hematological parameters and immunological response by the administration of aqueous extract of C. roseus leaves and probiotic in AIDR.
Hypothesis: It was assumed that aqueous extract of C. roseus and probiotic would improve the hematological profile and stimulate the immune response in diabetic rats.
Methodology: Twenty adult healthy rats (weighing 164-278g) were selected for experimental study. The rats were housed in clean metallic cages and divided in to four groups A, B, C and D. Five male rats were kept in Group A and B separately. Four female rats were kept in Group B and C separately. All the rats in each group is weighed and labeled. Rats were kept maintained under control environmental conditions of 24±5 ºC with 40-50% humidity, 12 hours light and dark photoperiod. Animals had free access to standard diet and water for the experimental trial of 21 days. Animal were fed with plane diet no. 25 used for rodents. Alloxan was used as a diabetogenic compound in a single dose of 150 mg/kg BW. Rats were fasted for12 hours, allowing only water access prior to the alloxan injection. After the estimation of fasting blood glucose the rats were rendered diabetic by injecting freshly prepared solution of alloxan (dissolving 600mg alloxan/ 4ml physiological saline) according to average body weight of each group which was calculated earlier. After 4 days of alloxan injection blood samples were obtained from tail vein of rat and hyperglycemia was confirmed by measuring fasting blood glucose level with glucometer. Rats with fasting blood glucose level higher than 150 mg/dl were considered as diabetic and used for further studies in present investigation. Blood samples were collected from the rats after 21 days of experimental trial. Rats were anaesthetized with chloroform in glass desiccators and blood sample was obtained through cardiac puncture using hypodermic needle and syringe. Blood samples were collected in EDTA containing vacutanors labeled with groups. 1.0 micro liter blood was separated from each group blood samples for the estimation of hematological parameters. Blood samples were then centrifuged at 3500 x g for 10 minutes to separate serum. Collected serum samples were then stored in labeled eppendrof at -20OC for further serological analysis.
Results: In our study the feed intake was not significantly influenced in treatment groups. But the body weights were significantly different in treatment groups as compared to treatment group. In blood biochemical analysis it was revealed that serum total protein level was significantly increased in group B and group C. Whereas serum albumin concentrations were not vary significantly among groups. However serum globulin level showed a trend towards significancy. The hematologic parameters were not affected by C. roseus and probiotic supplementation alone or in combination. The skin response to DNCB challenge also did not show significant results, which indicated that immune response was not varying among groups.
Conclusion: It is concluded from the results of present investigation that C. roseus and probiotic alone or in combination improved the body weight abnormality associated with diabetes but did not affect the feed intake. C. roseus was more effective in relation to body weight changes. However, C. roseus and probiotic both significantly increased the serum total protein and albumin level. Furthermore there were no significant effects showed by C. roseus and probiotic on immune response.
Availability: Items available for loan: UVAS Library [Call number: 1840,T] (1).
20.
Effect Of Supplementation Of Catharanthus Roseus And Probiotics On Blood Biochemical Profile In Alloxan
by Eshwa Dar | Prof. Dr. Ijaz Ahmad | Dr. Sehrish | Dr.Imtiaz Rubbani.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1846,T] (1).
21.
Response Of Probiotic Supplimentiation In Ethonal Treated Rats On Health Status And Boon Minerals.
by Basma Yousaf | Dr Muhammad Shahbaz Yousaf | Dr Hafza Zaneb | Prof Dr Habib Ur Rehman | FBS.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1877,T] (1).
22.
Seasonal Variations In Libido Semen Quality Freezability And Fertility Of Cholistani Bulls
by Syed Aamer mahmood | Prof. Dr. Ijaz ahmad | Dr. Hafsa zaneb | Prof. Dr. Habib Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1901,T] (1).
23.
A Study Of Antioxid Properties Of Green Tea And Black Tea In Insulin Resistant Diabetic Rats
by Jazba aiman | Prof. Dr. Ijaz ahmad | Dr. Ali ahmad | prof .Dr. Habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2020,T] (1).
24.
A Study On Antioxidant Properties Of Vitamin C And Vitamin E In Insulin Resistant Diabetic Rats
by Syeda javaria nadir | Prof. Dr. Ijaz ahmad | Dr. Ali Ahmad | Prof. Dr. Habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2028,T] (1).
25.
Single Or Combined Effects Of Probiotics And Gallic Acid Supplementation On Serum Biochemistry In Dexamethasone
by Fahad mahmood | Dr. Muhammad shahbaz yousaf | Dr. Hafsa zaneb | Prof. Dr. habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2045,T] (1).
26.
Effect Of Anti-Stressor Supplement On Physiological Indices And Gastrointestinal Development Of Broiler
by Muhammad umar minhas | Prof. Dr. Habib ur rehman | Dr. muhammad shahbaz yousaf | Dr. Sanaullah.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2046,T] (1).
27.
Study Of Electrophysiological Indices Of Median Nerve Conduction In Patients With Or Without Carpal Tunnel Syndrome
by Shafaq nazir | Prof. Dr. Habib ur rehman | Dr. Hafsa zaineb | Dr. shahbaz yousaf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2058,T] (1).
28.
Single And Combined Effects Of Curcuma Longa And Probiotics Supplementation On Zootechnical Parameters Serm
by Nazish saleem | Muhammad Shahbaz yousaf | Dr. Hafsa zaneb | Prof. Dr. Habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2059,T] (1).
29.
Effect Of Gallic Acid On Oxidative Stress In Alloxan-Induced Diabetic Rats
by Sadia yaseen | Dr. Muhammad quaid zaman | Dr. Muhammad Shahbaz yousaf | Prof. Dr. Azhar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2061,T] (1).
30.
Reaponse Of Lactobacillus Based Probiotics Supplementation On Serum Biochemical Metabolites And Hepatic Enzymes in Hypothyroid Mice
by Fareeha Ch | Dr. M.Shahbaz yousaf | Dr. Hafsa zaneb | Prof Dr. Habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2089,T] (1).
31.
Response Of Lactobacillus Based Probiotics Supplementation On Zootechnical Parameters Bone Health
by Hira Najeeb | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb | Prof. Dr. Habib ur Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2099,T] (1).
32.
Single And Combned Effect Of Turmeric And Probiotic Supplementation On Serum Lioid And Creatinine Profile In Alloxan Induced Diabetic Rats
by Salman Zafar | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb | Prof. Dr. Habib ur Rehman.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2106,T] (1).
33.
Antiulcer Activisty Of Malva Parviflora Leaf Extract Narr Sonhal On Ethanol Induced Gastric Ulcer In Rat
by Sumeera Anjum | Dr. Imtiaz Rabbani | Prof. DR. Habib-ur-Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2122,T] (1).
34.
Development And Evaluation Of Zinc Oxide Nanoparticles As Feed Additives In Broilers
by Sajid Khan Tahir | Dr. Muhammad Quaid Zaman | Dr. Saima | Prof Dr. Habib ur Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2129,T] (1).
35.
Gastroprotective Effect Of Grewia Optiva (Dhamna) Leaf Extract On Ethano; Induced Gastric Ulcer In Rats
by Saira Aslam | Dr Imtiaz Rabbani | Prof. DR. Habib-ur-Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2137,T] (1).
36.
Effect Of Supplementation Of Xylanase On Feed Efficiency And Serum Biochemistry In Broilers
by Abida Niazi | Dr. Muhammad Quaid Zaman | Dr. Hafsa Zaneb | Prof. Dr. Habib ur Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2156,T] (1).
37.
Effect Of Gallic Acid Supplementation On Zootechnical Parameters Serum Biochemistry And Immune Response In Alloxan Induced Diabetic Rats
by Nida Sahar | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa | Prof. Dr. Habib -ur- Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2162,T] (1).
38.
Impact Of Activated Clotting Time6 On Post Cardiopulmonary Bypass Blood Loss
by Adnan Haider | Prof Dr. Habib ur Rehman | Dr. Muhammad Shahbaz Yousaf | Dr. Nisar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 214Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2171,T] (1).
39.
Effect Of Supplementation Of Fructo-Oligosaccharides And Manno-Oligosaccharides On Serum Biochemical Parameters In High-Fat Fed Rats
by Anam Jeelani (2013-VA-593) | Dr. Muhammad Quaid Zaman | Prof. Dr. Habib ur Rehman | Dr. Nauman Zahid.
Material type: ; Literary form:
not fiction
Publisher: 2013Dissertation note: Obesity is a public health issue, leading to metabolic disorders usually associated with the formation of cardiovascular disorder, fatty liver diseases and diabetes type II (Dewulf et al. 2006). Many factors considered to emergence of dyslipidemia and obesity (Parekh et al. 2014). Imbalance between intake of energy and utilizing of energy cause obesity, it might be caused by various factors including food intake, lack of physical activities, modern life style inherited, hypertension, and type of diet (Arora and Sharma 2011).
Many species of micro-organisms occupied gastrointestinal tract (GIT). But in colon portion most important populations are existing and showing symbiosis relationship which helps in maintaining good health (Roberfroid et al. 2010).GIT microflora is participating in metabolic disorders and obesity as well, indicating the fatty animal and human having different gut microbiota composition as compared to slim ones (Sanz et al. 2010). Reductions in Bacteroidetes and rise of the relative abundance of Firmicutes have been associated with obesity (Parnell et al. 2011).
Bifidobacteria play a part in the development of obesity and its related co-morbidities (Delzenne et al. 2011).It was found that decreased number of Bifidobacteria at birth was associated with overweight later in childhood (Kalliomaki et al. 2008). Diabetes mellitus type II patients having very less amount of such type of bacteria as compared to non diabetic person (Wu et al. 2010). Man considered as Holobiont or super-organism because of in its composition only 10% of human cells while rest of 90% are micro-organisms (Lederberg 2000).
Prebiotics are short chain length carbohydrates (Hang et al. 2013), non-digestible food constituents which significantly effects on the person by enhancing the metabolic activity, growth and also induce the production of GIT microbiota (Manning and Gibson 2004) and induce the growth of beneficial microflora and overcome the pathogenic organism (Gibson et al. 1995). The small intestine not having the enzymes for the decomposition of prebiotics, finally they reach the large intestine (Strickling et al. 2000; Gibson and Roberfroid 1995) where they are utilized by the beneficial microbiota to produce volatile fatty acids (VFAs) (Yang et al. 2007), finally the pH of the brush border microenvironment goes down and reducing the pathogenic effects.
Generally, intestinal microbiota considered as important for health issues and immunomodulatory activities and food intake induce and stimulate the GIT (Rehman et al. 2007). Microbiota has significant roles in the host physiology, commencing from nutritional and metabolism, to infection and disease. Many evidences show, prebiotics in GIT stimulates many other systems as well for example lymphoid system (GALT). The expenditure of prebiotics can modulate defensive elements in GALT, secondary lymphoid tissues and blood circulation (Schley and Field 2007).
This selectivity was shown for bifidobacteria, which may be promoted by the ingestion of substances such as fructooligosaccharides (FOS) and inulin (Gibson et al. 1995), transgalactosylated oligosaccharides (Tanaka et al. 1983).
FOS is an important group of prebiotics, which induce the production of bacteria in lower intestinal tract (Hang et al. 2013). FOS are oligosaccharides that found in plants for example in garlic, onion, artichoke, chicory, asparagus, banana, and in different plants. FOS composed of linear units of fructose and link together by beta (2-1) linkage.2 to 60 fructose number units together and end on glucose. Small intestine does not having the property to digest and hydrolysed the dietary FOS. While it contain Glycosidases and until it will be in ceum and there is no structural change of it. Then in metabolism of gut microbiota FOS converted into small chain carbolic acid, L-lactate, Hydrogen, Carbondioxide and other compounds. It contain very significant properties while low sweetness, may be use as calorie free, non-carcinogenic and are considered as soluble edible fibre. Many important and beneficial physiological aspects such as rate of mineral absorption increases, reduction of phosphoplipds, total cholesterol and triglycerides. Many food products contain FOS infant formulas causes to their prebiotic effect induce the growth of non-pathogenic gut microbiota. Metabolism affects the feces nature as well as the speed of deposition, and dose of 4-15 g/day taken by a normal person definitely having low constipation rate, also assume as a major problem of growth in a society. (Sabater-Molina et al. 2009).
FOS with significantly effected on GIT microbiota,lowering the GIT infections, and also possessing a recognized bifidogenic effect. Normally, prebiotics compounds collected by the extraction of many plants and then hydrolysed by enzymatic activity of sucrose (Hang et al. 2013).
Manno-oligosaccharide (MOS) obtained from mannans present on the cell wall of a fungus Saccharomyces cerevisiae is a well recognized prebiotic that contain competitive attaching sites for many bacteria, that’s why having capacity for many ligands. Pathogens having the mannose-specific fimbriae adsorb to the MOS rather intestinal epithelial cells (Spring et al. 2000).
Idea of prebiotics introduced in 1995, considered as non-digestable and partially fermented oligosaccharides found in food elements. Their presence in GIT may lower the effect of disease in colon. That idea about Prebiotics is revisited and possible mechanisms are proposed. Many functional aspects related to metabolism of prebiotics are assumed in sense of reducing many disorders (Van Loo 2004).
It is assumed that prebiotics have effects on the immune system, as it stabilizes the intestine by enhancing the gut microflora especially lactobacilli and some selected strains, which may change can modify host innate and acquired defensive mechanisms and also help to save in respiratory infections (Hori et al. 2001). Main objective of our current study is to elucidate effects of MOS, FOS supplementation on production performance, physiological indices of health including blood glucose level, total cholesterol, triglycerides etc.
Availability: Items available for loan: UVAS Library [Call number: 2222-T] (1).
40.
Effect Of Citrullus Colocynthis On Serobiological Parameters In Alloxan Induced Diabetic Rats
by Farah Javed (2012-VA-398) | Dr. Muhammad Quaid Zaman | Dr. Imtiaz Rabbani | Prof. Dr. Asim Aslam .
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2015Dissertation note: Diabetes mellitus is one of the most common endocrine disorders affecting almost 25% of the world's population.The pretentious worldwide increase in the incidence of diabetes mellitus is posturing a huge health problem in both developed and developing countries. Diabetes mellitus is a metabolic disorder which is manifested by polyuria, polyphagia, polydipsia, hyperglycemia and dyslipidemia and is still one of the most leading causes of disability and death. Oral hypoglycemic agent and insulin are common treatment of diabetes but these treatments have prominent side effects. In the recent years the use ofherbal medicines has increased for the treatment of diabetes and fascinated the consideration ofmany researchers all over the world. Citrullus colocynthis is used commonly in different parts of the world for the treatment of a number of diseases including diabetes, jaundice, leprosy, cancer, asthma, bronchitis, joint pain and mastitis. In the present study I evaluate the anti-diabetic effects of roots of Citrullus colocynthis and also the dose dependent anti-diabetic effects of medicine in alloxan induced diabetic rats.
Twenty-five adult male rats were divided into five groups; Negative control, Positive control, and 3 groups for different treatment dose of roots of Citrulllus colocynthis (200 mg/kg body weight, BW), Citrulllus colocynthis (300 mg/kg BW) and Citrulllus colocynthis (500 mg/kg BW). Diabetes induction had done in four groups, other than negative control (normal saline injected), by subcutaneous administration of alloxan (120 mg/kg BW). Blood glucose level of rats reached above 250mg/dl considered as hyperglycemic. Treatment was given to all groups excluding control negativefor 21 days. Body weight of rats of all groups was recorded weekly. After completing 21 days of treatment with different doses of roots of Citrullus colocynthis blood samples were collected in fasting condition from rats of each group by cardiac punctureunder general anesthesia. Serum was collected from blood to measure serum glucose level, serum lipid profile, liver function test and renal function test.
Result data was analyzed by using SPSS software. Data was analyzed by using one-way ANOVA. The group differences were compared by the Duncan’s Multiple Range Test. Differences was considered significant at P < 0.05.
The obtained results showed that roots of Citrullus colocynthis has efficiency to control the diabetes mellitus by reducing serum glucose levels as well as the increasing dose decreased the serum glucose levels. Only 500mg/kg body weight dose is efficient to reduce the muscle wastage due to diabetes in alloxan induce diabetic rats. This dose also works tomaintain the serum ALT, AST, urea, creatinine, total cholesterol and triglycerides, HDL-C LDL-C levels.
The data obtained from this study also show the dose dependent anti-diabetic activity of medicine as the dose of 500mg/kg body weight is more effective to control the diabetes as compared to other two doses; 200mg/kg body weight and 300mg/kg body weight.
Availability: Items available for loan: UVAS Library [Call number: 2297-T] (1).
41.
Response Of Dietary Supplementation Of Β-Galacto-Oligosaccharides And Benzoic Acid On Growth Performance And Gastrointestinal Physiology In Broiler Chickens
by Muhammad Shahbaz Yousaf (2007-VA-538) | Prof. Dr. Habib ur Rehman | Prof. Dr. Juergen Zentek | Prof. Dr. Ijaz Ahmad | Prof. Dr. Kamran Ashraf.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Efficient poultry production depends upon a number of crucial factors that include optimum nutrient digestibility, bioavailability and maximum conversion into end products like meat and egg as well as low morbidity and mortality in the flock. In the past, antibiotics were used successfully as growth promoters in poultry and animal feed to improve production performance. However, it has become increasingly apparent that many problems are associated with the use of antibiotic growth promoters (AGPs); most importantly, the emergence of increasing numbers of microbes resistant to antibiotics. Therefore, AGPs were banned in the poultry as well as in the livestock industries of many countries. Due to withdrawal of AGPs, the poultry industry faced outbreak of diseases, high mortality, compromised growth performance and thus low profitability. Researchers and producers must, therefore, seek an alternate for AGPs that provide safety both for poultry and consumer health. Many potential candidates have subsequently been put forth and evaluated to replace AGPs that include prebiotics, probiotics, phytobiotics and organic acids. These AGPs replacers showed variable degree of success depending upon nutritional, managemental and hygienic conditions. Keeping in view the importance of AGP replacers, it was the purpose of this study to the growth promoting potential of β-galacto-oligosaccharides (β-GOS), a prebiotic, and benzoic acid (BA), an organic acid, in growing broilers.
In experiment-1, 200 day old Hubbard broiler chicks, were randomly divided into four treatment groups (n = 50) with five replicates (n = 10) in each group. Birds in different treatment groups were allotted four different types of diets. Birds in the control group (β-GOS-0) were given ad-libitum corn based basal diet, free of antimicrobials and coccidiostats, whereas birds in three other experimental groups were given the same basal diets supplemented with different concentrations of β-GOS i.e., 0.1% (β-GOS-1), 0.2% (β-GOS-2) and 0.5% (β-GOS-5) for a period of 35 days. Birds in all the experimental groups were vaccinated against the Newcastle disease virus (NDV) and infectious bursal disease virus by giving primary and booster doses according to the schedule. Moreover, birds were also sensitized against 2, 4-dinitrochlorobenzene for determination of cell mediated immunity (CMI). Body weights, feed intake and feed conversion efficiency were determined on a weekly basis. At the end of experiment, ten birds from each group were slaughtered to collect blood, organs and caecal digesta for determination of serum biochemical metabolites and humoral immunity, organ characteristics and selected caecal digesta bacteria, respectively. Data were analyzed by using analysis of variance and incase of significant F-value (P < 0.05), data were subjected to Tukey's-HSD post hoc test. Results demonstrated that growth performance and feed conversion efficiency (FCE) of β-GOS supplemented birds were improved (P < 0.05) in a dose dependent manner, with the highest body weights (P < 0.05) and improved FCE (P < 0.05) observed in the β-GOS-5 group. Relative weights of liver and pancreas were higher (P < 0.05) in the β-GOS-1 group. Likewise, CMI was also higher (P < 0.05) in the β-GOS-1 group. Concentrations of caecal Lactobacilli spp. were higher in the β-GOS-2 and β-GOS-5 groups. The serum biochemical metabolites, humoral immunity in terms of antibodies titres against NDV and Clostridia spp. and coliforms were not affected by dietary supplementation of β-GOS. In conclusion, dietary supplementation of 0.5% β-GOS conferred a better response in terms of growth performance, feed conversion efficiency and caecal Lactobacilli spp. in broilers.
In experiment-2, 120 day old male Cobb broiler chicks were randomly divided into three treatment groups (n = 40) with eight replicates (n = 5) in each group. Birds in different treatment groups were allotted three different types of diets. Birds in the control group (CON) were given ad-libitum maize based basal diets, free of antimicrobials and coccidiostats, whereas birds in two other experimental groups were given the same basal diets supplemented with two different types of BA i.e., 0.096% free BA (FBA) and 0.2% slow releasing BA (SBA) for a period of 35 days. Body weights, feed intake and feed conversion efficiency were determined on a weekly basis. At the end of the experimental period, birds from each group were slaughtered to collect digesta from the crop, proventriculus, gizzard, proximal small intestine, ileum and caecum for determination of benzoic acid, microbial metabolites, microbial populations and molecular microbial ecology. Data were analyzed by using analysis of variance and incase of significant F-value (P < 0.05), data were subjected to Tukey's-HSD post hoc test. Results revealed that overall growth performance, feed intake and FCE remained unchanged among all the treatment groups. Total short chain fatty acids were only increased (P < 0.05) in the gizzard by both types of BA supplementation. Likewise, D-lactate concentration was increased (P < 0.05) by both types of BA in crop digesta, whereas, L-lactate was only increased (P < 0.05) by SBA in crop digesta. Both types of BA increased (P < 0.05) the concentrations of L-lactate, D-lactate and acetic acid in gizzard, and D-lactate in proximal small intestine. Concentrations of i-butyric acid and i-valeric acid were decreased (P < 0.05) in caecal digesta by both types of BA, whereas, n-valeric acid was only decreased (P < 0.05) by SBA supplementation in caecal digesta. Concentrations of both types of BA in different segments of gastrointestinal tract were comparable. Concentrations of gizzard Lactobacilli spp. were higher in both the BA supplemented groups, whereas, L. johnsoni and L. reuteri concentrations were higher in the SBA group compared with the control group. Similarly, in the proximal small intestine both types of BA increased the population of Lactobacilli spp., L. reuteri, L. amylovorus and Enterococci spp. Ileal concentrations of Lactobacilli spp., L. johnsoni, L. amylovorus and S. alactolyticus were higher (P < 0.05) in SBA group, whereas, L. reuteri was increased (P < 0.05) both by the FBA and SBA supplementations. Microbial populations of crop and caecum remained unchanged among all the treatment groups. Results of ileal molecular microbial ecology analysis revealed that evenness was higher (P < 0.05) in both the BA supplemented groups, whereas, the Sorensen index (P < 0.05) and the Renkonen index (< 0.5) were different only in the SBA group. However, caecal microbial ecology analysis revealed no changes among all the treatment groups. In conclusion, FBA and SBA supplementations produced similar responses in terms of microbial metabolites production with no effect on overall growth performance and feed conversion efficiency. However, SBA conferred some advantages over FBA in terms of increased bacterial concentrations, especially some species of the Lactobacilli as well as S. alactolyticus, and improved ileal molecular microbial ecology.
Availability: Items available for loan: UVAS Library [Call number: 2310-T] (1).
42.
In Vitro Effects Of Zinc Oxide Nanoparticles On Electrophysiological Indices Of Jejunal Mucosa Of Laying Hens
by Wajeeha Mehmood (2013-VA-899) | Prof. Dr. Habib-Ur-Rehman | Dr. Muhammad Quaid Zaman | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Zn is a vital micro-nutrient in living body and food industries have been using Zn as feed additive in animal diets. Previous studies presented that the performance of organic Zn differ from inorganic Zn due to higher bioavailability of organic Zn than that of inorganic Zn, but the addition of organic Zn in animal diet is costly. Therefore, to fulfil animal’s requirements, Zn is added in excess than the normal requirement. This can leads to environmental pollution when stool with excess Zn is released outside. Also the excess dietary Zn may cause other trace elements and vitamins. NPs are regarded to be highly absorbed into the gastrointestinal systems than larger particles. ZnO NPs in lesser quantity instead of bulk-sized ZnO, can be a better option. Different zinc salts affects the intestinal ion transport. There is less information about ZnO NPs effects on intestinal transport. The current study is established to study the in vitro effects of ZnO NPs on electrophysiological properties and its flux across jejunal mucosa of laying hens. Twelve White Leghorn laying hens were procured from a local commercial farm, kept in a shed and provided with feed and water ad libitum. After a week the birds were killed and jejunum was then removed, washed and conveyed in ice-cold oxygenated buffer to the laboratory within 5 minutes. Four segments were taken out of jejunum and designated with four groups (Control, ZnO 70 µM, ZnO NPs 70 µM and ZnO 35 µM). Each of the segment were mounted on separate UC. After mounting, twenty minutes were given to the tissues for equilibration under open circuit and then short-circuited by clamping the voltage at 0 mV. After getting the base line, the ZnO NPs and ZnO were added in separate chambers. The ZnO NPs or ZnO was added to the mucosal compartment of the Ussing chamber where its response was noted. Glucose was added after the incubation of approximately twenty to twenty five minutes. The electrical response was measured as the peak response obtained two minutes after the addition of glucose. The change in Isc (∆Isc) was estimated after addition of ZnO, ZnO NPs and glucose. Presentation of data was done as means ± S.E.M. The data was analyzed using One-Way Analysis of variance. The group differences were compared by the Post Hoc Tukey Test. Differences were considered significant at P < 0.05. The result showed that there was an obvious decrease in Isc of tissue treated with ZnO (Group II) but a slight increase in Isc was observed when segments treated with ZnO NPs at 70 µM and 35 µM concentration (Group III and IV). Statistically, the mean ∆Isc values of Group II was significantly different from control, Group III and IV. While Group III and IV were not significantly different from control. Also, no change existed between Group III and IV. A significant decline in glucose induced Isc was observed in tissue of Group II as compare to control, Group III and IV. There was a slight non-significant decline in glucose induced Isc when treated with ZnO NPs at 70 µM and 35 µM concentration. In conclusion, ZnO NPs are not showing any significant interference with the ionic and glucose transport and can be used as a food supplement to fulfil Zn demands. On the other hand, ZnO suppress Cl- ion secretion and glucose transport in vitro. Further confirmation of these fact needs more studies with more decreased dose of ZnO NPs.
Availability: Items available for loan: UVAS Library [Call number: 2439-T] (1).
43.
The Effect Of Feeding Of Physically, Chemically And Biologically Treated Rice Husk On Serum Minerals In Sheep
by Sohrab Ahmad (2014-VA-529) | Prof. Dr. Habib ur Rehman | Dr. Muhammad Shahbaz Youaf | Dr. Rahat Naseer.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Rice is the second major cereal crop cultivated in Pakistan. The rice crop‘s residual parts, i.e. straw and husk are used as an animal feed. To enhance the nutritive value and digestibility of these residues different treatments are used, including Physical, Chemical and Biological treatments to enhance animal performance. In the present study, the effect of Physical, Chemical and Biological treatment of rice husk was observed on the serum mineral profile which is also an indicator of an animal’s performance.
Thirty six adult sheep were divided into six groups. Negative control group 1, positive control 2, physically treated group 3 acid treated rice feeding group 4, alkali treated rice husk group 5, and biologically fermented rice husk group 6. Each group received 20% treated rice husk along with the routine ration. Blood was collected at day 60 and serum was separated, which was analyzed for minerals by using atomic absorption specrophotometrery and flame photometry.
Data was analyzed using SPSS software. One-way ANOVA was applied on data to observe among the group difference while group differences were compared by Duncan’s Multiple Range Test. Differences were considered significant at P < 0.05.
The results revealed that serum level of zinc was significantly higher (p < 0.05) in group 6 compared to group 4 and 1 but similar to group 2 and other treatment groups (figure 1). However, we found a tendency towards significance (p=0.056) in serum calcium level, which was higher in group 5 compared to other treatment groups and group 1 but was found similar to group 2. The serum sodium level also showed a trend towards significance (p=0.072) in group 2, 3 and 5 compared to group 6. Serum iron and potassium level were found non-significant (p<0.05) in all treatments groups compared to group 1 and 2. Blood hemoglobin level was found significantly higher (P=0.013) in control positive group compared to control negative group, 3, 4, 5and was similar to group 6. The platelet count was found in a tendency towards significance (P=0.062), higher in all treatment groups compared to the negative control group. The hematological parameters, i.e. RBC, WBC, MCV, MCH, and MCHC were found non-significant (p< 0.05) in both the control groups and all treatment groups.
Concluding the above discussion, we suggest that fermented rice husk feeding may have better effect on the animal n terms of increase in minerals retention and hemoglobin level in the blood which will cause an increase in the metabolic activities and hence growth of the animal.
Availability: Items available for loan: UVAS Library [Call number: 2465-T] (1).
44.
Effects Of Ethanolic And Aqueous Extracts Roots And Leaves Of Catharanthus Roseus On Serum Biochemical Parameters In Broilers
by Kiran Sabir (2014-VA-05) | Prof. Dr. Habib ur Rehman | Dr. Muhammad ShahbazYousaf | Mr.Shahid Abbas.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The study was designed to checkthe effects of supplementation of C. roseus in various concentrations (0.05%, 0.1% and 0.2%) as an alternative to AGPs on various physiological indices of birdshealth. Antibiotic growth promoters (AGPs) have been used in poultry farms as a drug resistance and drug residues. Now, there is a need to look for safe alternative for AGPs. Catharanthusroseushaving the properties of antimicrobial, antiviral, antifungal, antioxidant and growth promoter.
Total 260 birds weretaken and divided into the 13 groups. Each group was further sub-divided into four replicates with 5 birds in each replicate. The birds were supplemented with basal diet (Group A) and all other groups were supplemented with diet of aqueous extract of Cathrathusroseus at the concentration of 0.05%, 0.1% and 0.2% in the Groups B, C, D, E, F, G, H, I, J, K,L and M for 35 days trial. During the trial period, the birds were vaccinated with Newcastle disease and Infectious Bursal Disease Vaccine (IBDV). Blood was also collected on day 35 to determine the concentration of various physiological indices.
Statistical analysis was conducted by using Statistical Packages For Social Sciences (SPSS Version 20 USA). To analyzed the data one way ANOVA was used.Post hoc test was carried out using tukey.
The results of glucose was found significantly higher (P<0.05) in group H compared to group K and M but similar to group B, C, D, E, F, G, I and J and M. The results of total cholesterol was found significantly higher (P<0.05) in group A, B, D, G, and I compared to group F and J but similar to group C, E, H, K, L and M.The serum Triglycerides concentrationwas no significantly (P<0.05) difference in all treatment groups compared to the control group.The serum ALT concentration was found significantly higher (P<0.05) in group F, G L and M than control group but similar in Groups B, C D E, H, I, J and K.The serum AST concentration was significantly higher (P<0.05) in group K and significantly lower in Groups B,C,I,J,L,M but similar in Groups D,E,F,G,H,A .
It was concluded that Catharanthusroseus can be use as a cheaper, easiest and quickly available source as an alternative of antibiotic in the poultry.
Availability: Items available for loan: UVAS Library [Call number: 2504-T] (1).
45.
In Vitro Study Of The Effect Of Menthol On Electrophysiological Indices Of Isolated Rumen Epithelium In Goat
by Najma Khan (2014-VA-506) | Dr. Imtiaz Rabbani | Prof. Dr. Habib ur Rehman | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Ammonia is product of protein metabolism and its reabsorption occur by non-selective cation channels across rumen epithelium otherwise it will not be available as non-protein nitrogen source and excreted out causing hazard to environment. At physiological pH, ammonia is present as NH4+ in rumen and its transport leads to electrophysiological changes. It was reported that addition of menthol to the isolated rumen epithelium of cattle cause electrophysiological changes, primarily linked to the ammonium transport suggesting presence of non-selective cation channels. Addition of cation channel modulators such as menthol to diet can enhance absorption of nitrogen in the rumen. Scarce data was available for rumen epithelium of goats which has a large contribution in livestock population and has a great potential for mutton market revealing effect of cation channel modulators on electrophysiology and ammonium transport. Objective of this study was to investigate the effect of menthol on electrophysiological changes and its link to ammonia transport in the isolated rumen epithelium of goats.
Hypothesis
Menthol may affect the electrophysiology of the isolated rumen epithelium of goats and enhance ammonia transport across rumen epithelium.
Methodology
Ten goats of uniform age and weight were selected and slaughtered in a local slaughter house. Reticulorumen was removed from the abdominal cavity and cleaned in a standard transport buffer solution. Serosal layer of rumen was removed and mounted in the Ussing chamber. Rumen epithelium was incubated with transport buffer solution and after 20 minutes replaced with ammonia containing buffer solution (10mM and 40mM respectively)
46
and changes in bioelectric parameters were observed. Menthol in concentration of 1mM was added to mucosal side and electrophysiological parameters like Isc and Gt were observed. Moreover, pH of serosal side was checked after menthol treatment at time intervals 20, 60 and 90 minutes respectively to assess ammonia transport.
Results
Menthol affected the electrophysiology of isolated rumen epithelium of goats by increasing short circuit current due to increase in ammonia concentrations in the rumen. Menthol addition in 40mM NH4Cl containing solution significantly increased current (p value = 0.00). ΔIsc was also significant between the groups containing 10 and 40mM NH4Cl containing solution. Change in potential difference was significant after addition of menthol in both groups (P = 0.00). Conductance increased significantly by increasing concentration of ammonia (p value = 0.03). PH of serosal side decreased, suggesting possible NH4+ uptake, while on mucosal side increase in pH was observed.
Statistical analysis
Data were analyzed by using completely randomized ANOVA followed by post-hoc test LSD. Level of significance was set at 0.05.
Conclusion
Menthol treatment affects the electrophysiological indices of isolated rumen epithelium of goat presumably by opening non-selective cation channels and enhances ammonium transport across rumen epithelium increasing recycling of nitrogen. This may leads to efficient protein utilization by the animals. Availability: Items available for loan: UVAS Library [Call number: 2558-T] (1).
46.
Evaluation Of Cardioprotective Effect Of Citric Acid On Serum Biochemical Profile Against Isoproterenol Induced Myocardial Infarction In Rabbits
by Aasma Shabbir (2014-VA-525) | Prof. Dr. Habib ur Rehman | Dr. Muhammad Shahbaz Yousaf | Dr. Nisar Ahmad .
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Isoproterenol is a drug which is used to treat heart attack, congestive heart failure, shock and certain types of irregular heartbeat. In addition to this, it is also employed during the process of anesthesia to avoid the constriction of airways. Isoproterenol is a synthetic catecholamine which produced myocardial infarction because of production of cytotoxic free radicals. Citric acid is water soluble and is most important antioxidant and enzyme cofactor. Recent evidence suggests that citric acid possess antioxidant activity.
The aim of this study was to optimize a supplement at which citric acid can act as cardio protector against isoproterenol and also to evaluate its effect on level of CK-MB, serum glucose, serum creatinine, urea, uric acid, triglycerides, total cholesterol, HDL-C, AST, ALT, ALP.
Forty rabbits were selected and housed in the experimental shed of the Department of physiology, University of Veterinary and Animal Sciences, Lahore. Before the arrival of rabbits, the shed was cleaned and fumigated. The rabbits were divided randomly in to five groups, each with eight replicates (n=8 in each group). Animals were treated by following treatment plan; Group 1: (Negative Control) Animals received normal saline 1ml orally for 14 days. Group 2: (Positive Control) Animals received normal saline 1ml orally for 14 days and then myocardial infarction induced on 15th day. Group 3: Animals received citric acid 250 mg/kg body weight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day. Group 4: Animals received citric acid 500 mg/kg bodyweight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day. Group 5: Animals received citric acid 750 mg/kg body weight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day.
At the end of the experiment, rabbits were slaughtered to collect blood samples for serum biochemical analysis (CK-MB, lipid profile, LFT’s, RFT’s, serum glucose). Data was analyzed by one way analysis of variance using SPSS software (SPSS Inc. version 20, Chicago, Illinois). The group differences were studied by using Duncan’s multiple range tests. The P value <0.05 was considered as significant. Data was presented as mean ± SD.
Results showed that the level of CK-MB, creatinine, urea, HDL-C, ALT were found significant (P<0.05) in rabbits compared with the control. While there was no significant effect found on serum glucose, uric acid, total cholesterol, triglycerides, AST, ALP in all the experimental groups compared with control.
From our study we have concluded that supplementation of citric acid has cardioprotective effect against isoproterenol induced myocardial infarction in rabbits. It shows significant effect on CK-MB, HDL-C, ALT, urea and creatinine. While there was no significant effect found on serum glucose, uric acid, total cholesterol, triglyceride, AST, ALP.
Availability: Items available for loan: UVAS Library [Call number: 2703-T] (1).
47.
Estimation Of Serum Homocysteine Level In Patients With Ischemic Or Hemorrhagic Stroke
by Iqra Ikhlaq (2014-VA-810) | Prof. Dr. HabiburRehman | Dr. Ahsan Numan | Dr. Muhammad ShahbazYousaf | Dr. Hafsa Zainab.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Stroke is the second major cause of deaths and common cause of disabilities worldwide. Increased level of homocysteine is considered as a critical but treatable risk factor for stroke. Homocysteine has many harmful effects on vascular system including thrombosis induction, increased in oxidative stress, stimulating mitogenesis and impaired endothelial function. Stroke patients with elevated homocysteine level have more frequently developed multiple infarctions and cerebral microangiopathy. In the present study, the serum homocysteine level was measured in Pakistani acute stroke patients.
Subjects selected for the study was divided in to two groups. Group 1 (Control) having 30 healthy individuals and in Group 2 (Patients) having 68 stroke patients. Both of the groups, controls and patients were sex and gender matched. The stroke subtypes (ischemic and hemorrhagic) was diagnosed by neurologists based on the neuroimaging (CT or MRI). Neurologic functions assessment was based on (NIHSS) Stroke Score. Biochemical parameters i.e. total cholesterol,triglycerides, high density lipoprotein, low density lipoprotienand serum homocysteine was measured by using commercially available kits at the end of the experiment.
The data was analyzed by using SPSS software. Student's t-test was applied on data to compare serum homocysteine concentrations and other continuous variable of patients and control groups. A chi-square test was used to analyze the qualitative findings. Differences will be considered significant at p < 0.05.Comparison among stroke cases and controls for homocysteine and other stroke risk factors were performed by using binary logistic regression analysis. The odds ratios (OR) and 95 confidence intervals (95% CI) were also estimated.
The results revealed that serum homocysteine level was significantly higher (p=0.001) in stroke patients. Other risk factor of stroke were also significantly high in stroke patients as hypertension (p=0.000), diabetes mellitus (p=0.009) and smoking (p=0.008). Clinical data revaeled that Systolic blood pressure (mmHg) (p=0.000), diastolic blood pressure (mmHg) (p=0.006), cholesterol (p=0.003), triglyceride (p=0.008), LDL cholesterol level (p=0.006) and serum creatinine level (p=0.010) was also significantly raised in stroke patients.
Concluded that in stroke patients, the measurement of serum homocysteine level may be effective and useful to get a clearer image about patients, condition and this is beneficial for disease prevention and management. Treating hyperhomocystenemia may be helpfull in formulating strategies in reducing stroke incidence and its complications in Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 2721-T] (1).
48.
Electrophysiological Evalution Of Patients Suffering From Juvenile Epilepsy
by Masuma Amin (2014-VA-526) | Prof. Dr. Habib ur Rehman | Dr. Ahsan Numan | Dr. Muhammad Shahbaz Yousaf | Ms. Amina Chughtai.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Epilepticogenic seizures are episodes of excessive, abnormal and repeatsynchronous neuronal activity in the brain. Seizures can be accompanied by clinical neurological symptoms as alterations in consciousness and abnormal body movements.These epileptic activities are causing impermanent disturbance in brain an message signals became mixed up and it results in epileptic seizure. The electrophysiological changes occur in epileptic seizure in the brain and it can be diagnosed by the EEG which is an electrical presentation of impulses on a paper. The electrophysiological evaluation of children with epilepsy was made by the EEG machine. There are many risk factors contributing to the occurrence of epilepsy including cousin marriages, positive family history and affected sibling. Different types of seizures are studied which included Generalized tonic clonic, Myoclonic and tonic clonic.
This study was a cross sectional study in which 50 epileptic children and 25 control subjects with no epilepsy were studied. The age of the patients was between 4-18 years divided into 4 groups regardless of gender in this study conducted in Services Hospital Lohore.EEG was performed and history has been taken, a questionnaire was filled by parents and clinical examination was done.
This study showed that there are electrophysiological changes in epileptic seizure and the wave changes exhibit in epilepsy (p=0.03) which shows significant results .Similarly the history of family with epilepsy has significant relation with occurrence of epilepsy(p= 0.037). The cousin marriage are also contributing factors in occurrence of epilepsy as it has link with genes and it run into families showing the significant association (p= 0.040).The sibling are also affected if there is presence of epilepsy in any one of the child in the family(p=0.020). Hague severity scale was applied that reveals that the severity of epilepsy occurred as the number of scale increases and it affects the daily activity of the individuals.Chi Square test was applied to analyze the Electrophysiological changes in epilepsy while Binary Logistic Regression was applied to analyze the different contributing factors in prevalence and occurrence of epilepsy.
Availability: Items available for loan: UVAS Library [Call number: 2719-T] (1).
49.
Effects Of Mannanoligosaccharides Feeding On Selected Mineral Profile In Post-Weaned Goat Kids
by Tasneem Kausar (2014-VA-556) | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Habib ur Rehman | Dr. Saif ur Rehman Kashif.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Minerals play a pivotal role in kids’ growth and development. Minerals deficiency in young age has lifelong consequences. It is necessary to maintain adequate level of minerals in kids body to match their requirements. This can be achieved either by supplementing diet with minerals or by enhancing their absorption. Mannan-oligosaccharides supplementation can enhance minerals concentration in liver, muscles, blood and kidney by enhancing their absorption in gastrointestinal tract.
Ten healthy goat kids were selected for study purpose to evaluate effects of prebiotics supplementation on minerals profile of serum, liver, muscles and kidneys. These kids were divided into 2 groups. One group (control group) was on normal basal diet other the experimental group was fed with diet supplemented with 1 g mannan-oligosaccharides. The kids were slaughtered on day 75 and sampling was done. Clear non hemolysed sera were separated for serum mineral analysis. Samples from liver muscles and kidneys cut into small pieces and dried. Wet digestion of samples done and upto 50 ml of solution of each sample was made for spectrophotometry. Calcium levels are analyzed by flame photometry and atomic absorption spectrophotometer was used to evaluate levels of copper, zinc and iron.
Results obtained are statistically analyzed by applying students-t test and presented as mean ± SE and considered significant at P < 0.05. The results of the study, to evaluate the relationship between mannan-oligosaccharides supplementation and minerals absorption, were not significant. MOS has not any significant effects on minerals profile in goat kids.
Availability: Items available for loan: UVAS Library [Call number: 2711-T] (1).
50.
Effects Of Supplementation Of Zinc Sulphate And Lactobacillus Based Probiotic On Oxidative Status And Liver Health In Broilers
by Wishah Bilal (2015-VA-04) | Dr. Sajid Khan Tahir | Prof. Dr. Habib ur Rehman | Dr. Sanaullah Iqbal.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Zinc is considered as one of the essential nutrients as well as a dietary supplement required for animal metabolism. Use of probiotics enhances mucosal immunity of intestine i.e. it prevents the mucosa against the toxins formed by pathogenic microorganisms. Combined effect of zinc and probiotics may show a better response than their single effect on the oxidative status, biochemical profile and thyroid hormones in broilers. Our research was aimed to determine the single as well as combined effects of zinc and probiotic on oxidative status, liver enzymes, serum glucose, total serum proteins, thyroid hormones, urea, uric acid and creatinine levels in broilers.
A total of 192 day old broiler chicks were randomly selected and divided into six groups: Control, Zn30 (30mg Zn/ kg diet), Zn60 (60mg Zn/ kg diet), Pro (0.1g/ kg Protexin®), Com30 (30mg Zn/ kg diet + 0.1g/ kg Protexin®) and Com60 (60mg Zn/ kg diet + 0.1g/ kg Protexin®). Birds were raised under standard management conditions and were given free access to feed and water for a period of 42 days. For biochemical analysis, blood was collected in falcon tubes after slaughtering. For serum separation, blood was centrifuged at 1500 rpm for 10 min and stored at ˗20˚C. The parameters that were studied included: serum catalase, MDA, glucose, total protein, albumin, globulin, ALT, AST, T3, T4, urea, uric acid and creatinine. Data was analyzed by applying one-way ANOVA using SPSS. Group differences were compared by Duncan Multiple Range Test and were considered significant at P<0.05.
According to our study catalase activity was found significantly lower (P<0.05) and MDA level was found significantly higher (P<0.05) in the control group as compared to the supplemented groups. The birds fed with a combination of zinc (60 mg/kg) and protexin showed highest (P<0.05) catalase activity and lowest (P<0.05) MDA level in comparison to other supplemented groups. A significant increase (P<0.05) in serum proteins was obseved with all the supplementations in comparison to the control group. Only the combination of zinc (30 mg/kg) and protexin did not show the expected result. In our research we found that protexin significantly increased (P<0.05) serum T3 level as compared to other supplemented groups while zinc (30 mg/kg) showed significantly lower (P<0.05) serum T3 level than the control group. On the other hand, serum T4 concentration significantly increased (P<0.05) with both zinc (60 mg/kg diet) and its combination with protexin. Our research depicted that combination of zinc (60mg/kg diet) and protexin significantly increased (P<0.05) urea while both the combinations showed significantly increased (P<0.05) uric acid and (P<0.05) decreased creatinine concentrations in serum. There was no significant (P<0.05) change in serum glucose, ALT and AST concentrations in birds given zinc and probiotic supplementations.
It can be concluded that protexin and zinc supplementations are beneficial in improving biochemical parameters of broilers, yet their synergistic effects need to be studied further.
Availability: Items available for loan: UVAS Library [Call number: 2802-T] (1).
